Liu Chi-Ying, who was born in 1905 in Suzhou, Jiangsu, graduated from Jinling University with a bachelor of science in biology in 1929. After graduation, Liu worked at the Bureau of Entomology of Jiangsu province and taught in the Department of Biology at Zhejiang University. In 1933, Liu came to the University of Minnesota and received his master of science degree in entomology in 1935. During his time studying at the University, he was part of the Sigma Xi Honorary Society and the Gamma Sigma Delta Honorary Society. Liu was awarded a gold key from each society.
Upon returning to China, Liu taught at Guangxi University, Zhejiang University, and Academy of Military Medical Sciences successively. In addition, Liu served as the chairman of the boards for the Hangzhou and Shanghai Entomology Societies. He also served as the deputy chair of the board for the China Entomology Society and was the deputy editor-in-chief for a journal called Acta Zootaxonomica Sinica.
Liu was one of the founders of the Chinese Journal of Vector Biology and Control. He won the first and second prizes for Awards of Military Science and Technology Advancement, and the second prize for National Natural Science. Liu served as the editor for the following important publications: The Taxonomy of Siphonapterology in China, China Vegetable Insects, The Study of Insects Control, and Medical Entomology.